• Vacunas a células dendríticas (CDs) en sistemas experimentales. Experimentos anteriores demostraron que la inmunización con CDs produce escasa migración a los ganglios linfáticos. Hemos demostrado que en el sitio de vacunación se forman estructuras linfoides terciarias.
• Vacunas a células dendríticas (CDs) en sistemas humanos. La vacunación con CDs cargadas con células alogeneicas irradiadas aumentó la sobrevida de pacientes con melanoma. Estamos analizando cómo incrementar la inmunogenicidad de dichas vacunas.
• En 2009 la ANMAT aprobó un Estudio Clínico de Fase II/III sobre 108 pacientes de melanoma, utilizando la vacuna CSF 470, randomizada contra interferon (Instituto Alexander Fleming).
• Investigación de células stem tumorales. Se caracterizarán células con capacidad clonogénica a partir de líneas celulares de melanoma y cáncer colorectal. Además, estudiaremos nuevos Ags por cDNA arrays. Se procurará establecer un perfil Ag característico de CST. Produciremos una biblioteca de fagos de anticuerpos de llama contra Ags de melanoma. Investigaremos vías de señalización características de CST a fin de diseñar antagonistas específicos.
Para desarrollar los proyectos arriba mencionados, se utilizarán técnicas de RT-PCR, real-time PCR, ingenería genética, biología molecular, inmunohistoquímica, microscopía confocal, citometría de flujo, cultivo de tejidos, análisis de lípidos, ELISPOT, construcción de biblioteca de fagos, Micro-Disección Laser (MDL) y antibody arrays.

Mac Keon S, Ruiz MS, Gazzaniga S, Wainstok R. Dendritic cell-based vaccination in cancer: therapeutic implications emerging from murine models. Frontiers in Immunology 2015. doi: 10.3389/fimmu.2015.00243. Pubmed

Arriaga JM, Greco A, Mordoh J, Bianchini M. Metallothionein 1G and zinc sensitize human colorectal cancer cells to chemotherapy. Mol Cancer Ther 2014. doi: 10.1158/1535-7163.MCT-13-0944. Pubmed

Roberti MP, Juliá EP, Rocca YS, Amat M, Bravo AI, Loza J, Coló F, Loza CM, Fabiano V, Maino M, Podhorzer A, Fainboim L, Barrio MM, Mordoh J, Levy EM. Overexpression of CD85j in TNBC patients inhibits Cetuximab-mediated NK-cell ADCC but can be restored with CD85j functional blockade. Eur J Immunol 2015. doi: 10.1002/eji.201445353. Pubmed

Madorsky Rowdo FP, Barón A, Urrutia M, Mordoh J. Immunotherapy in Cancer: A Combat between Tumors and the Immune System; You Win Some, You Lose Some. Frontiers in Immunology 2015.  doi: 10.3389/fimmu.2015.00127. Pubmed

Ruiz MS, Mac Keon S, Campisano S, Bravo AI, Gazzaniga S, Wainstok R. CD207⁺ cells recruitment to the vaccination site and draining lymph nodes after the administration of DC-Apo/Nec vaccine in mice. Vaccine 2014. doi:10.1016/j.vaccine.2014.01.025. Pubmed

Roberti MP, Arriaga JM, Bianchini M, Quintá HR, Bravo AI, Levy EM, Mordoh J, Barrio M. Protein expression changes during human triple negative breast cancer cell line progression to lymph node metastasis in a xenografted model in nude mice. Cancer Biology and Therapy. 2012. doi: 10.4161/cbt.21187 PubMed

Rocca YS, Roberti MP, Arriaga JM, Amat M, Bruno L, Pampena MB, Huertas E, Sánchez Loria F, Pairola A, Bianchini M, Mordoh M and Levy EM. Altered phenotype in peripheral blood and tumor-associated natural killer cells from colorectal cancer patients . Innate Immunity 2012 doi: 10.1177/1753425912453187 PubMed

Barrio MM, Abes R, Colombo M, Pizzurro G, Boix C, Roberti MP, Gélizé E, Rodriguez-Zubieta M, Mordoh J, Teillaud JL. Human Macrophages and Dendritic Cells Can Equally Present MART-1 Antigen to CD8(+) T Cells after Phagocytosis of Gamma-Irradiated Melanoma Cells. PLoS One. 2012;7(7):e40311. Epub 2012 Jul 2. PubMed

Pizzurro GA, Madorsky Rowdo FP, Pujol-Lereis LM, Quesada-Allué LA, Copati AM, Roberti MP, Teillaud JL, Levy EM, Barrio MM, Mordoh J. High lipid content of irradiated human melanoma cells does not affect cytokine-matured dendritic cell function. Cancer Immunol Immunother. 2012 Jun 22. [Epub ahead of print] PubMed

Arriaga JM, Bravo IA, Bruno L, Morales Bayo S, Hannois A, Sanchez Loria F, Pairola F, Huertas E, Roberti MP, Rocca YS, Aris M, Barrio MM, Baffa Trasci S, Levy EM, Mordoh J, Bianchini M. Combined metallothioneins and p53 proteins expression as a prognostic marker in patients with Dukes stage B and C colorectal cancer. Hum Pathol. 2012 Apr 17. [Epub ahead of print] PubMed

Madorsky Rowdo Florencia P., Lacreu María Laura, Mordoh José. Melanoma vaccines and modulation of the immune system in the clinical setting: bulding from new realities. Frontiers in Immunology. 2012 PubMed

Campisano S.,Mac Keon S., Gazzaniga S., Ruiz S., Dodes Traian M., Mordoh J., Wainstok R. Vaccine. Anti-melanoma vaccinal capacity of CD11c –positive and-negative populations present in GM-CSF cultures derived from murine bone marrow precursors. 2012 PubMed

Aris M, Barrio MM and Mordoh J, Lessons from cancer immunoediting in Cutaneous Melanoma. Clinical and Developmental Immunology, 2012, en prensa. PubMed

Arriaga JM, Levy EM, Bravo AI, Bayo SM, Amat M, Aris M, Hannois A, Bruno L, Roberti MP, Loria FS, Pairola A, Huertas E, Mordoh J, Bianchini M. Metallothionein expression in colorectal cancer: relevance of different isoforms for tumor progression and patient survival. Hum Pathol. 2012 Feb;43(2):197-208. Epub 2011 Aug 4. PubMed

Levy EM, Roberti MP, Mordoh J. Natural Killer Cells in Human Cancer: From Biological Functions to Clinical Applications. J Biomed Biotechnol. 2011;2011:676198. Epub 2011 Apr 26. Review. PubMed

Roberti MP, Barrio MM, Bravo AI, Rocca YS, Arriaga JM, Bianchini M, Mordoh J, Levy EM. IL-15 and IL-2 increase Cetuximab-mediated cellular cytotoxicity against triple negative breast cancer cell lines expressing EGFR. Breast Cancer Res Treat. 2011 Nov;130(2):465-75. Epub 2011 Feb 10 PubMed

Aris M, Rodríguez Zubieta M, Colombo M, Arriaga JM, Bianchini M, Alperovich M, Bravo AI, Barrio MM, Mordoh J. MART-1 and gp100 expressing and non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro. 2011 J Invest Dermatol. 132(2):365-74. PubMed

Powlesland AS, Barrio MM, Mordoh J, Hitchen PG, Dell A, Drickamer K, Taylor ME. Glycoproteomic characterization of carriers of the CD15/Lewis x epitope on Hodgkin's Reed-Sternberg cells. BMC Biochem. 2011 Mar 24;12:13. PubMed

Mac Keon S., Gazzaniga S., Mallerman J., Bravo AI., Mordoh J., Wainstok R. Vaccination with dendritic cells charged with apoptotic/necrotic b16 melanoma induces the formation of subcutaneous lymphoid tissue. Vaccine 2010. Nov 29; 28(51): 8162-8 PubMed

Aris M, Barrio MM and Mordoh J. Cutaneous Melanoma: a test field for immunotherapy and a medical challenge. Current Cancer Therapy Reviews.2010 6(3): 229-242.

Levy, E.M., Sycz, G., Arriaga, J.M., Barrio, M.M., von Euw, E.M., Morales, S.B., González, M., Mordoh, J., Bianchini, M. Cetuximab-mediated cellular cytotoxicity is inhibited by HLA-E membrane expression in colon cancer cells.Innate Immun. 15: 91-100  (2009).     PubMed

Powlesland, A.S., Hitchen, P.G., Parry, S., Graham, S.A., Barrio, M.M., Elola, M.T., Mordoh, J., Dell, A., Drickamer, K., Taylor, M.E. Targeted glycoproteomic identification of cancer cell glycosylation. Glycobiology. 19: 899-909 (2009).     PubMed