4. Regulación traduccional de HIF y la función de Musashi
Durante la búsqueda de sitios de unión de miRNAs que realizamos en las RNAs mensajeros de las subunidades de HIF, encontramos secuencias consenso de reconocimiento del represor traduccional Musashi. Hemos demostrado que Musashi controla la traducción de HIF (Bertolin et al. 2016) y actualmente estamos caracterizando el papel fisiológico del eje Musashi-HIF en el desarrollo y la diferenciación celular de Drosophila. aaaaaaaaaaaaaGándara L, Durrieu L, Behrensen C, Wappner P. A genetic toolkit for the analysis of metabolic changes in Drosophila provides new insights into metabolic responses to stress and malignant transformation. Sci Rep. 2019 Dec 27;9(1):19945. PubMed
DM Blanco-Obregon, MJ Katz, L Durrieu, L Gándara, P Wappner. Context-specific functions of Notch in Drosophila blood cell progenitors. bioRxiv 682658; Junio 25, 2019. doi: https://doi.org/10.1101/682658
Riesgo-Escovar JR, Mayor R, Wappner P, Yan I. MoD Special issue on "Developmental Biology in Latin America". Mech Dev. 2018 Dec;154:1. PubMed
Markolovic S, Zhuang Q, Wilkins SE, Eaton CD, Abboud MI, Katz MJ, McNeil HE, Leśniak RK, Hall C, Struwe WB, Konietzny R, Davis S, Yang M, Ge W, Benesch JLP, Kessler BM, Ratcliffe PJ, Cockman ME, Fischer R, Wappner P, Chowdhury R, Coleman ML, Schofield CJ. The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases. Nat Chem Biol. 2018 Jul;14(7):688-695. PubMed
Gándara L, Wappner P. Metabo-Devo: A metabolic perspective of development. Mech Dev. 2018 Feb 21. pii: S0925-4773(18)30001-7. Review PubMed
Melani, M., Valko, A., Romero, N.M., Aguilera, M.O., Acevedo, J.M., Bhujabal, Z., Perez-Perri, J., de la Riva-Carrasco, R.V., Katz, M.J., Sorianello, E., D'Alessio, C., Juhász, G., Johansen, T., Colombo, M.I., Wappner, P. Zonda is a novel early component of the autophagy pathway in Drosophila. Mol Biol Cell. 2017 Sep 13. doi: 10.1091/mbc.E16-11-0767. PubMed
Perez-Perri, J.I., Dengler, V.L., Audetat, A.K., Pandey, A., Bonner, E.A., Urh, M., Mendez, J., Daniels, D.L., Wappner, P., Galbraith, M.D., Espinosa, J.M. The TIP60 complex is a conserved coactivator of HIF1A. Cell Reports. 2016 Jun 28;16(1):37-47. PubMed
De Lella Ezcurra, A.L., Bertolin, A.P., Kim, K., Katz, M.J., Gándara, L., Misra, T., Luschnig, S., Perrimon, N., Melani, M., Wappner, P. miR-190 Enhances HIF-Dependent Responses to Hypoxia in Drosophila by Inhibiting the Prolyl-4-hydroxylase Fatiga. PLoS Genet. 2016 May 25;12(5):e1006073. PubMed
Bertolin, A.P., Katz, M.J., Yano, M., Pozzi, B., Acevedo, J.M., Blanco-Obregón, D., Gándara, L., Sorianello, E., Kanda, H., Okano, H., Srebrow, A., Wappner, P. Musashi mediates translational repression of the Drosophila hypoxia inducible factor. Nucleic Acids Res. 2016 May 3 PubMed
Katz, M.J., Gandara, L., De Lella Ezcurra, A.L., Wappner, P. Hydroxylation and translational adaptation to stress: some answers lie beyond the STOP codon. Cell Mol Life Sci. 2016 Feb 13. Review PubMed
Simpson, P.D., Eipper, B.A., Katz, M.J., Gandara, L., Wappner, P., Fischer, R., Hodson, E.J., Ratcliffe, P.J., Masson, N. Striking Oxygen Sensitivity of the Peptidylglycine α-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem. 2015 Oct 9;290(41):24891-901. PubMed
Katz, M.J., Acevedo, J.M., Wappner, P. Growing with the wind. Ribosomal protein hydroxylation and cell growth. Fly (Austin). 2014;8(3):153-6. Review PubMed
Singleton, R.S., Liu-Yi, P., Formenti, F., Ge, W., Sekirnik, R., Fischer, R., Adam, J., Pollard, P.J., Wolf, A., Thalhammer, A., Loenarz, C., Flashman, E., Yamamoto, A., Coleman, M.L., Kessler, B.M., Wappner, P., Schofield, C.J., Ratcliffe, P.J., Cockman, M.E. OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation. Proc Natl Acad Sci U S A. 2014 Feb 18. [Epub ahead of print] PubMed
Katz, M.J., Acevedo, J.M., Loenarz, C., Galagovsky, D., Liu-Yi, P., Pérez-Pepe, M., Thalhammer, A., Sekirnik, R., Ge, W., Melani, M., Thomas, M.G., Simonetta, S., Boccaccio, G.L., Schofield, C.J., Cockman, M.E., Ratcliffe, P.J., Wappner, P. Sudestada1, a Drosophila ribosomal prolyl-hydroxylase required for mRNA translation, cell homeostasis, and organ growth. Proc Natl Acad Sci U S A. 2014 Feb 18. [Epub ahead of print] PubMed
Galagovsky, D., Katz, M.J., Acevedo, J.M., Sorianello, E., Glavic, A., Wappner, P. The Drosophila insulin degrading enzyme restricts growth by modulating the PI3K pathway in a cell autonomous manner. Mol Biol Cell 2014 Jan 15. [Epub ahead of print] PubMed
De Lella Ezcurra, A.L., Bertolin, A.P., Melani, M., Wappner, P. Robustness of the hypoxic response: another job for miRNAs? Dev Dyn. 241(12):1842-8 (2012) Review PubMed
Perez-Perri, J.I., Acevedo, J.M., Wappner, P. Epigenetics: new questions on the response to hypoxia. Int J Mol Sci. 12(7):4705-21 (2011). Review PubMed
Acevedo, J.M.; Centanin, L., Dekanty, A., Wappner, P. Oxygen sensing in Drosophila: multiple isoforms of the prolyl hydroxylase fatiga have different capacity to regulate HIFalpha/Sima. PLoS One 5(8), e12390 (2010). PubMed
Centanin, L., Gorr, T.A., Wappner, P. Tracheal remodelling in response to hypoxia. J Insect Physiol. 56: 447-454 (2010). PubMed
Dekanty, A., Romero, N.M., Bertolin, A.P., Thomas, M.G., Leishman, C.C., Perez-Perri, J.I., Boccaccio, G.L.,Wappner, P. Drosophila genome-wide RNAi screen identifies multiple regulators of HIF dependent transcription in hypoxia. PLoS Cenet. 6: e1000994 (2010). PubMed
Irisarri, M., Lavista-Llanos, S., Romero, N.M., Centanin, L., Dekanty, A., Wappner, P. Central role of the oxygen-dependent degradation domain of Drosophila HIFalpha/Sima in oxygen-dependent nuclear export. Mol Biol Cell. 20: 3878-3887(2009). PubMed
Centanin, L., Dekanty, A., Romero, N., Irisarri, M., Gorr, T.A., Wappner, P. Cell autonomy of HIF effects in Drosophila: Tracheal cells sense hypoxia and induce terminal branch sprouting. Dev Cell. 14: 547-558 (2008). PubMed
Romero, N.M., Irisarri, M., Roth, P., Cauerhff, A., Samakovlis, C., Wappner, P. Regulation of the Drosophila hypoxia-inducible factor alpha sima by CRM1-dependent nuclear export. Mol Cell Biol. 28: 3410-3423 (2008). PubMed
Rojas, D.A., Munizaga, D.A., Centanin, L., Antonelli, M., Wappner, P., Allende, M.L., Reyes, A.E. Cloning of hif-1a and hif-2a and mRNA expression pattern during development in zebrafish. Gene Expr Patterns. 7: 339-345 (2007). PubMed
Romero, N.M., Dekanty, A., Wappner, P. Cellular and developmental adaptations to hypoxia: a Drosophila perspective. Methods Enzymol. 435: 123-144. (2007). Review PubMed
Gorr, T.A., Gassmann, M., Wappner, P. Sensing and responding to hypoxia via HIF in model invertebrates. J. Insect. Physiol. 52: 349-364 (2006). Review PubMed
Centanin, L., Ratcliffe, P.J., Wappner, P. Reversion of lethality and growth defects in Fatiga oxygen-sensor mutant flies by loss of Hypoxia-Inducible Factor-alpha/Sima. EMBO Rep. 6: 1070-1075 (2005). PubMed
Dekanty, A., Lavista-Llanos, S., Irisarri, M., Oldham, S., Wappner, P. The insulin-PI3K/TOR pathway induces a HIF-dependent transcriptional response in Drosophila by promoting nuclear localization of HIF-alpha/Sima. J. Cell. Sci. 118: 5431-5441 (2005). PubMed
Muzzopappa, M., Wappner, P. Multiple roles of the F-box protein Slimb in Drosophila egg chamber development.Development 132: 2561-2571(2005). PubMed