Molecular Basis of Plant Development

José M. Estevez - Fundación Instituto Leloir


Molecular Basis of Plant Development

Both pollen tubes and root hairs are single plant cells that play crucial roles in the survival of plants either in fertilization and seed formation or the acquisition of nutrients and water. Their cell expansion is polarized and directional. This study project proposes to study the polarized cell expansion of both cell types in Arabidopsis thaliana as experimental model. Currently, we have the following research lines that explores the molecular regulation of polarized cell expansion in root hairs and pollen tubes: (1) NADPH oxidases (NOX) that produce Reactive Oxygen Species ROS and Ca+2 transporting systems that regulates the flux of Ca+2 ions. Both are major regulators of cell growth; (2) apoplastic peroxidases (PERs) that interlinks cell wall proteins Extensins (EXTs) and impact on the cell wall structure; (3) Transcription factors (FTs) involved in the regulation of ROS production.
  1. Molecular mechanism that allows apoplastic peroxidases (PERs) to regulate the assembly of glycoprotein Extensins (EXTs). Role of receptor like kinases with an extracellular EXT-type domain (PERKs). Identification of downstream components of PERK sensing pathway.
  1. Molecular mechanisms that modulates polarized cell growth in root hairs and pollen tubes. Identification and characterization of NADPH oxidases (NOXs) and Ca+2 transporting systems.
  1. Identification of relevant Transcription Factors (FT) in root hairs and pollen tubes growth linked to ROS production.
Molecular components that regulate polarized cell growth in pollen tubes and root hairs. The active form of NOXs generate apoplastic ROS, which activates Ca+2 transporting system that raises the levels of this ion in the cytoplasm and promotes the activation of NOXs. The apoplastic Ca2+ associated to pectins is released and transported. Changes in pH are also triggered by H+ transport by H+-ATPases in the apoplast. 1-3 indicates the main research aspects we are currently following in order to understand the molecular basis of the cell expansion process. CDPKs= Ca+2 dependent protein Kinases; EXTs= Extensins; HG= HomoGalacturonans; NOX= NADPH oxidase; K= kinase domain; PER= Peroxidases; PERKs= Proline-Rich Extensin Receptor like Kinase. TFs= Transcription Factor.

Mangano S., Denita-Juarez, S.P., Marzol, E.. Borassi, C., and José M. Estevez. 2018. High auxin and high Pi impact on RSL2 and ROS-linked root hair growth in Arabidopsis. Frontiers in Plant Science. PubMed

Mangano S.*, Martínez Pacheco, J.*, et al. J. M. Estevez. 2018. How does pH fit in oscillating polar growth?. Trends in Plant Sciences. 23(6):479-489. PubMed

Marzol, E.*, Borassi, C.*, et al. J. M. Estevez. 2018. Filling the gaps to solve the EXTENSIN puzzle. Molecular Plant. 11 (5): 645-658. PubMed

Cagnola J.I., et al., Estevez, J.M., J.J. Casal. 2018. Reduced ARABINOGALACTAN PROTEIN abundance anticipates stress-induced kernel abortion in field crops of maize. Plant Cell & Environment. PubMed

Sede, A.R, C. Borassi et al. José M. Estevez*and Jorge P. Muschietti*. 2018. Multiple Arabidopsis pollen extensin-like (PEXs) proteins are needed for cell wall integrity during pollen tube growth. FEBS Letters. .  *Autor correspondiente.PubMed

Arata P.X. et al. Estevez J.M.* and Ciancia M*. 2017. Sulfated polysaccharides in the freshwater green macroalga Cladophora surera not linked to salinity adaptation. Front. Plant Sci. 8:1927. *Autor correspondiente. IF 4.3.PubMed

Eliana Marzol et al. J.M. Estevez. 2017. RSL4 Takes Control: Multiple Signals; One Transcription Factor. Trends in Plant Science. 10.1016/PubMed

E. Marzol, C. Borassi, S.P. Denita-Juárez, S. Mangano, J. M. Estevez. 2017. Trends in Plant SciencePUBMED.

Mangano S., S.P. Denita-Juarez, Choi, Marzol E. et al. & J.M. Estevez. 2017. PNAS 10.1073/pnas.1701536114. PubMed

Borassi C., Sede A.S., Mecchia, M., Muschietti J.P., Estevez J.M. 2016. J. Exp. Bot. 76(2): 477-487.PubMed

Velasquez S.M., Jurgen Klein-Vein & J.M. Estevez. 2016. Auxin and cellular elongation. Plant Physiology. 170: 1206-1215. . IF 7.3 PubMed

Velásquez S.M., et al. & J.M. Estevez. 2015. Low sugar is not always goods: impact of specific O-glycan defects on tip-growth in Arabidopsis. Plant Physiology. 168(3): 808-13. PubMed

Velásquez S.M. et al. & Estevez J.M. 2015.Complex regulation of Prolyl-4-hydroxylase 5 impacts root hair expansion. Molecular Plant 8:734-746. PubMed

José Manuel Estevez
Principal Investigator -
Scientific Report

Silvina Mangano
Assistant Researcher CONICET

Javier Martínez Pacheco
Postdoctoral fellow

Cecilia Borassi
Ph.D. Student - CONICET

Eliana Marzol
Ph.D. Student - CONICET

Diana Rodriguez Garcia
Ph.D. Student - AGENCIA

Ana Rocio Sede
Ph.D. Student - CONICET - Co-Dirigido con Jorge Muschietti, INGEBI

Julián Garcia Bossi
Ph.D. Student - CONICET - Co-Dirigido con Jorge Muschietti, INGEBI

Yossmayer Rondon
Ph.D. Student - AGENCIA

Mariana Carignani Sardoy
Predoctoral Fellow

Bin Li
co-dirigido con Feng Yu, Hunan University

Victoria Berdion Gabarian