Bioquímica y Biología Estructural
Dra. Silvia Borkosky
Cargo actual Desde 01/2014 Investigador Asistente. Laboratorio de Estructura-Función e Ingeniería de Proteínas. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA) CONICET – Fundación Instituto Leloir (FIL), Buenos Aires, Argentina. Tema de trabajo: “Estudio del efecto anti-carcinogénico de la proteína E2 de HPV16 en cuanto a su interacción con blancos celulares y virales”. Postdoctorado 05/2009 – 10/2012 Postdoctoral Scientist. Division of Characterization of Tumorviruses, Research Program Infection and Cancer, DKFZ – German Cancer Research Center, Heidelberg, Alemania. Tema de trabajo: “Rol de los virus Torque teno en esclerosis múltiple y cáncer” Doctorado 03/2005 – 03/2009 Estudiante de Doctorado (Daigakuin-sei). Título obtenido: Doctor of Philosophy (Ph. D). Major: Biopathological Science. Título de tesis: “Frequent deletion of ING2 locus at 4q35.1 associates with advanced tumor stage in head and neck squamous cell carcinoma”. Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japón. Entrenamiento en diagnóstico histopatológico de lesiones orales 04/2004 – 03/2009 Estudiante de Doctorado (Daigakuin-sei). Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japón. Supervisión: Prof. Dr. Hitoshi Nagatsuka, D.D.S., Ph. D., Oral Pathologist.
Borkosky SS, Peralta-Martínez R, Armella-Sierra A, Esperante SA, Lizárraga L, García-Pardo J, Ventura S, Sánchez IE, de Prat Gay G. Experimental kinetic mechanism of p53 condensation-amyloid aggregation. Biophysical Journal. 2025;124(10):1658-1673. https://doi.org/10.1016/j.bpj.2025.04.009 Research article PubMed
Borkosky SS, Fassolari M, Campos-León K, Rossi HA, Salgueiro M, Pascuale CA, Peralta-Martínez R, Gastón K, de Prat Gay G. Biomolecular condensation of the human papillomavirus E2 master regulator with p53: Implications in viral replication. Journal of Molecular Biology. 2023;435(16):167889. https://doi.org/10.1016/j.jmb.2022.167889 Research article PubMed
Salgueiro M, Camporeale G, Visentin A, Arán M, Pellizza L, Esperante SA, Corbat A, Grecco H, Sousa B, Esperón R, Borkosky SS, de Prat Gay G. Molten globule driven and self-downmodulated phase separation of a viral factory scaffold. Journal of Molecular Biology. 2023;435(16):168153. https://doi.org/10.1016/j.jmb.2023.168153 Research article PubMed
Armella-Sierra A, Loureiro ME, Esperante SA, Borkosky SS, Gallo GL, de Prat Gay G, López N. Nuclease activity of the Junín virus nucleoprotein C-terminal domain. Viruses. 2023;15(9):1818. https://doi.org/10.3390/v15091818 Research article PubMed
Visentin A, Demitroff N, Salgueiro M, Borkosky SS, Uversky VN, Camporeale G, de Prat Gay G. Assembly of the tripartite and RNA condensates of the respiratory syncytial virus factory proteins in vitro: Role of the transcription antiterminator M2-1. Viruses. 2023;15(6):1329. https://doi.org/10.3390/v15061329 Research article PubMed
Esperante SA, Alvarez-Paggi D, Salgueiro M, Desimone MF, de Oliveira GAP, Arán M, García-Prado J, Aptekmann AA, Ventura S, Alonso LG, de Prat Gay G. A finely tuned interplay between calcium binding, ionic strength and pH modulates conformational and oligomerization equilibria in the Respiratory Syncytial Virus Matrix (M) protein. Archives of Biochemistry and Biophysics. 2022;731:109424. https://doi.org/10.1016/j.abb.2022.109424 Research article PubMed
González-Foutel NS, Glavina J, Borcherds WM, Safranchik M, Barrera-Vilarmau S, Sagar A, Estaña A, Barozet A, Garrone NA, Fernández-Ballester G, Blanes-Mira C, Sánchez IE, de Prat Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB. Conformational buffering underlies functional selection in intrinsically disordered protein regions. Nature Structural & Molecular Biology. 2022;29(8):781-790. https://doi.org/10.1038/s41594-022-00811-w Research article PubMed
López N, Camporeale G, Salgueiro M, Borkosky SS, Visentín A, Peralta-Martínez R, Loureiro ME, de Prat Gay G. Deconstructing virus condensation. PLoS Pathogens. 2021;17(10):e1009926. https://doi.org/10.1371/journal.ppat.1009926 Review PubMed
Leroy L, Barbosa JARG, de Prat Gay G, Polikarpov I, Pinheiro CB. The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1. Proteins. 2020;88(1):106-112. https://doi.org/10.1002/prot.25773 Research article PubMed
Álvarez-Paggi D, Esperante SA, Salgueiro M, Camporeale G, de Oliveira GAP, de Prat Gay G. A conformational switch balances viral RNA accessibility and protection in a nucleocapsid ring model. Archives of Biochemistry and Biophysics. 2019;671:77-86. https://doi.org/10.1016/j.abb.2019.06.005 Research article PubMed
Álvarez-Paggi D, Lorenzo JR, Camporale G, Montero L, Sánchez IE, de Prat Gay G, Alonso LG. Topology dictates evolution of regulatory cysteines in a family of viral oncoproteins. Molecular Biology and Evolution. 2019;36(7):1521-1532. https://doi.org/10.1093/molbev/msz085 Comparative Study PubMed
Conci J, Álvarez-Paggi D, de Oliveira GAP, Pagani TD, Esperante SA, Borkosky SS, Arán M, Alonso LG, Mohana-Borges R, de Prat-Gay G. Conformational isomerization involving conserved proline residues modulates oligomerization of the NS1 interferon response inhibitor from the syncytial respiratory virus. Biochemistry. 2019;58(26):2883-2892. https://doi.org/10.1021/acs.biochem.8b01288 Research article PubMed
Leroy LMD, Barbosa JARG, de Prat Gay G, Polikarpov I, Pinheiro CB. The structure of the extended E2 DNA-binding domain of the Bovine Papillomavirus-1. Proteins. 2019;88(1):106-112. https://doi.org/10.1002/prot.25773 Research article PubMed
Esperante SA, Álvarez-Paggi D, Salgueiro M, de Prat Gay G. Mechanism of tetramer dissociation, unfolding, and oligomer assembly of Pneumovirus M2-1 transcription antiterminators. ACS Omega. 2018;3(11):14732-14745. https://doi.org/10.1021/acsomega.8b01600 Research article PubMed
Glavina J, Román EA, Espada R, de Prat Gay G, Chemes LB, Sánchez IE. Interplay between sequence, structure and linear motifs in the adenovirus E1A hub protein. Virology. 2018;525:117-131. https://doi.org/10.1016/j.virol.2018.08.012 Research article PubMed