Bioquímica y Biología Estructural
Dra. Silvia Borkosky
Cargo actual Desde 01/2014 Investigador Asistente. Laboratorio de Estructura-Función e Ingeniería de Proteínas. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA) CONICET – Fundación Instituto Leloir (FIL), Buenos Aires, Argentina. Tema de trabajo: “Estudio del efecto anti-carcinogénico de la proteína E2 de HPV16 en cuanto a su interacción con blancos celulares y virales”. Postdoctorado 05/2009 – 10/2012 Postdoctoral Scientist. Division of Characterization of Tumorviruses, Research Program Infection and Cancer, DKFZ – German Cancer Research Center, Heidelberg, Alemania. Tema de trabajo: “Rol de los virus Torque teno en esclerosis múltiple y cáncer” Doctorado 03/2005 – 03/2009 Estudiante de Doctorado (Daigakuin-sei). Título obtenido: Doctor of Philosophy (Ph. D). Major: Biopathological Science. Título de tesis: “Frequent deletion of ING2 locus at 4q35.1 associates with advanced tumor stage in head and neck squamous cell carcinoma”. Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japón. Entrenamiento en diagnóstico histopatológico de lesiones orales 04/2004 – 03/2009 Estudiante de Doctorado (Daigakuin-sei). Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japón. Supervisión: Prof. Dr. Hitoshi Nagatsuka, D.D.S., Ph. D., Oral Pathologist.
Borkosky Susana S, Fassolari M, Campos-León K, Hugo Rossi A, Salgueiro M, Antonela Pascuale C, Peralta Martínez R, Gaston K, de Prat Gay G. Biomolecular condensation of the human papillomavirus E2 master regulator with p53: Implications in viral replication. J Mol Biol. (2023 in Press). doi: 10.1016/j.jmb.2022.167889. PubMed
Salgueiro M, Camporeale G, Visentin A, Aran M, Pellizza L, Esperante SA, Corbat A, Grecco H, Sousa B, Esperón R, Borkosky SS, de Prat-Gay G. Molten globule driven and self-downmodulated phase separation of a viral factory scaffold. J Mol Biol. (2023 in Press). doi: 10.1016/j.jmb.2023.168153. PubMed
Visentin A, Demitroff N, Salgueiro M, Borkosky SS, Uversky VN, Camporeale G, de Prat-Gay G. Assembly of the Tripartite and RNA Condensates of the Respiratory Syncytial Virus Factory Proteins In Vitro: Role of the Transcription Antiterminator M2-1. Viruses. 15(6):1329 (2023). doi: 10.3390/v15061329. PubMed
Esperante SA, Alvarez-Paggi D, Salgueiro M, Desimone MF, de Oliveira GAP, Arán M, García-Prado J, Aptekmann AA, Ventura S, Alonso LG, Prat-Gay G. A finely tuned interplay between calcium binding, ionic strength and pH modulates conformational and oligomerization equilibria in the Respiratory Syncytial Virus Matrix (M) protein. Arch Biochem Biophys. 731:109424 (2022). doi: 10.1016/j.abb.2022.109424. PubMed
González Foutel NS, Glavina J, Borcherds WM, Safranchik M, Barrera-Vilarmau S, Sagar A, Estaña A, Barozet A, Garrone NA, Fernandez-Ballester G, Blanes-Mira C, Sánchez IE, de Prat-Gay G, Cortés J, Bernadó P, Pappu RV, Holehouse AS, Daughdrill GW, Chemes LB. Conformational buffering underlies functional selection in intrinsically disordered protein regions. Nat Struct Mol Biol. 29(8):781-790 (2022). doi: 10.1038/s41594-022-00811-w. PubMed
Lopez N, Camporeale G, Salgueiro M, Borkosky SS, Visentín A, Peralta-Martinez R, Loureiro ME, de Prat-Gay G. Deconstructing virus condensation. PLoS Pathog. 17(10):e1009926 (2021). doi: 10.1371/journal.ppat.1009926. PubMed Review
Leroy L, Barbosa JARG, de Prat-Gay G, Polikarpov I, Pinheiro CB. The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1. Proteins. 88(1):106-112 (2020). doi: 10.1002/prot.25773. PubMed
Alvarez Paggi D, Esperante SA, Salgueiro M, Camporeale G, de Oliveira GAP, Prat Gay G. A conformational switch balances viral RNA accessibility and protection in a nucleocapsid ring model. Arch Biochem Biophys. 671:77-86 (2019). doi: 10.1016/j.abb.2019.06.005. PubMed
Alvarez-Paggi D, Lorenzo JR, Camporale G, Montero L, Sánchez IE, de Prat Gay G, Alonso LG. Topology dictates evolution of regulatory cysteines in a family of viral oncoproteins. Mol Biol Evol. 36(7):1521-1532 (2019). doi: 10.1093/molbev/msz085. PubMed
Conci J, Alvarez-Paggi D, de Oliveira GAP, Pagani TD, Esperante SA, Borkosky SS, Aran M, Alonso LG, Mohana-Borges R, Prat-Gay G. Conformational Isomerization Involving Conserved Proline Residues Modulates Oligomerization of the NS1 Interferon Response Inhibitor from the Syncytial Respiratory Virus. Biochemistry. 58(26):2883-2892 (2019). 10.1021/acs.biochem.8b01288. PubMed
Leroy LMD, Barbosa JARG, de Prat-Gay G, Polikarpov I, Pinheiro CB. The structure of the extended E2 DNA-binding domain of the Bovine Papillomavirus-1. Proteins. 88(1):106-112 (2019). doi: 10.1002/prot.25773. PubMed
Esperante SA, Alvarez-Paggi D, Salgueiro M, Prat Gay G. Mechanism of Tetramer Dissociation, Unfolding, and Oligomer Assembly of Pneumovirus M2-1 Transcription Antiterminators. ACS Omega. 3(11):14732-14745 (2018). doi:10.1021/acsomega.8b01600. PubMed
Glavina J, Román EA, Espada R, de Prat-Gay G, Chemes LB, Sánchez IE. Interplay between sequence, structure and linear motifs in the adenovirus E1A hub protein. Virology. 525:117-131 (2018). doi: 10.1016/j.virol.2018.08.012. PubMed
Molina IG, Esperante SA, Marino Buslje C, Chemes LB, de Prat-Gay G. Cooperative RNA recognition by a viral transcription antiterminator. J Mol Biol. 430(6):777-792 (2018). doi: 10.1016/j.jmb.2018.01.018. PubMed
Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H. Structure and stability of the Human respiratory syncytial virus M(2-1) RNA-binding core domain reveals a compact and cooperative folding unit. Acta Crystallogr F Struct Biol Commun. 74(Pt1):23-30 (2018). doi:10.1107/S2053230X17017381. PubMed