Research area
Plant biology

MOLECULAR BASIS OF PLANT DEVELOPMENT
Research
Our laboratory studies the molecular bases of cell size regulation in plant cells. A better understanding of the impact of root cell development and nutrient absorption, as well as root anchoring in the soil when nutrients are one of the most important limiting factors for improving seed and fruit productivity. All our research aims to answer the following questions: How are RALFs-FER downstream components of the signaling cascades linked to extracellular signals such as nutrients/water and temperature? Functions of RALF (Rapid Alkalinization Factor) peptides that control the signaling pathway involving FERONIA (FER) kinase-like receptors during root hair growth. How are the intracellular Ca2+ reservoirs coordinated to contribute to the cytoplasmic Ca2+ gradient in these growing polar cells? Functions of ACA and ECA Ca2+-ATPases in the polar growth of root hairs and pollen tubes. How are temperature conditions and nutrient deficiency decoded as extracellular stimuli in a defined transcriptional network in these individual plant cells? Identify the transcriptional network controlled by transcription factors activated at low temperature in root hair cells using RNA-seq and ChIP-seq approaches.
Skills & tools
To understand the molecular mechanisms that regulate how plant cells expand, we use a very wide range of experimental approaches: 1. Techniques of Genetics and Molecular Biology. Isolation of single and multiple mutants (T-DNA and CRISPR-CAS lines, RNAi and amiRNA), inducible and overexpressing lines. Analysis of co-expressed genes. Global characterization of the plant genome using RNA-seq, CHIP-seq techniques, etc. 2. Cell Biology Methods. Confocal microscopy for proteins with fluorescent tags at the tissue and individual cell level. Protein reporters. Fluorescent biosensors (Yellow Cameleon YC3.6 and Hyper, roGFP) for measuring ROS and Ca+2 in real time. Protein-protein interaction techniques (e.g., FRET and BiFC). Co-localization methods. 3. Biochemical Methods. Pharmacological inhibition of enzymes. Isolation of small molecules. Glycobiology of plant cells. Enzymatic activity.
Collaboration interests
- RNA-seq, DAP-seq and ChIP-seq sequencing with a particular focus on root biology.
- ROS and Ca+2 Biosensors confocal microscopy.
- Developing of more tolerant crops: Tomato, Medicago and Rice.
Selected publications
- ESTEVEZ, José M. Two antagonistic gene regulatory networks drive Arabidopsis root hair growth at low temperature linked to a low-nutrient environment. New Phytologist, 2025, p. 1.l
- PACHECO, Javier Martínez, et al. Cell surface receptor kinase FERONIA linked to nutrient sensor TORC signaling controls root hair growth at low temperature linked to low nitrate in Arabidopsis thaliana. New Phytologist, 2023, vol. 238, no 1, p. 169-185.l
- PACHECO, Javier Martinez, et al. Apoplastic class III peroxidases PRX62 and PRX69 regulate ROS-homeostasis and cell wall associated extensins linked to root hair growth at low-temperature in Arabidopsis thaliana. bioRxiv, 2021, p. 2021.08. 20.456256.

Principal investigator
Jose Manuel Estevez, PhD
- root hair growth
- nutrient sensing
- calcium signaling
- transcriptional networks
- plant development