Plant Molecular Biology

Pablo D. Cerdán - Fundación Instituto Leloir


Plant Molecular Biology

As sessile organisms, plants have developed exquisite sensory systems which allow them to perceive daily environmental changes and to anticipate seasonal changes as well. Light is one of the most important environmental cues and affects development during the whole life cycle. First, even a very short exposure to light triggers germination. As seedlings emerge from soil, they are exposed to increasing light intensities which promote deetiolation, a process that includes cotyledon opening, expansion and greening, and the inhibition of hypocotyl elongation. During vegetative developent, light is very important to shape plant arquitecture. Plants elongate stems in the presence of shading or potentially shading competitors and position their leaves to maxiize light capture. Light is also important to regulate senescence; shaded leaves tend to senesce earlier. When reproductive stages approach, light again is essential. The length of the light period (photoperiod) is perceived by plants to anticipate seasonal changes, regulating processes as flowering and growth cessation (bud set). The perception of light is accomplished by several families of photoreceptors. In the model system Arabidopsis thaliana, four different families of photoreceptors were already characterized, the phytochromes which perceive red and far-red light (5 members, phyA to phyE), two cryptochromes (cry1 and cry2) which perceive UV-A and blue light, two phototropins (phot1 and phot2) which mediate the phototropism induced by blue-light . three LOV domain photoreceptors (fkf, ztl and lkp2) and a UV-B photoreceptor. These photoreceptors show specific and overlapping roles, forming a signaling transduction network that regulate the above mentioned processes. In our laboratory, we are interested in understanding how light acts through photoreceptors to regulate flowering and deetiolation and how these processes are integrated with development. More specifically, we use genetic and molecular approaches to study the interactions among photoreceptors, the integration of signals at the transcriptional level, through the Mediator complex, and the establishment of cellular memory of transcriptional status by epigenetic mechanisms.
Under Construction

Sabrina Iñigo, Mariano J. Alvarez, Bárbara Strasser, Andrea Califano & Pablo D. Cerdán (2012). PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis. The Plant Journal 69: 601-612. PubMed

Sabrina Iñigo, Adrián N. Giraldez, Joanne Chory & Pablo D. Cerdán (2012) Proteasome-Mediated Turnover of Arabidopsis MED25 Is Coupled to the Activation of FLOWERING LOCUS T Transcription. Plant Physiology, 160: 1662-1673. PubMed

Sabrina Iñigo, Mariana R. Barber, Maximiliano Sanchez-Lamas, Francisco Iglesias and Pablo D. Cerdán (2011). The photomorphogenic signal: an essential component of photoautotrophic life. En: Advances in Photosynthesis - Fundamental Aspects. ISBN 978-953-307-928-8. Book edited by: Dr. Mohammad Mahdi Najafpour, Department of Chemistry, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran.

Pablo D Cerdán (2011). Regulation of flowering time by light. In: The Flowering Process and its Control in Plants: Gene Expression and Hormone Interaction, 2011: 123-155 ISBN: 978-81-308-0436-1 Editor: Mac Yaish

Sabrina E. Sanchez, Ezequiel Petrillo, Esteban J. Beckwith, Xu Zhang, Matias L. Rugnone, C. Esteban Hernando, Juan C. Cuevas, Micaela A. Godoy Herz, Ana Depetris-Chauvin, Craig G. Simpson, John W.S. Brown, Pablo D. Cerdán, Justin O. Borevitz, Paloma Mas, M. Fernanda Ceriani, Alberto R. Kornblihtt & Marcelo J. Yanovsky (2010). A methyl transferase links the circadian clock to the regulation of alternative splicing Nature : 468: 112-116. PubMed

Bárbara Strasser, Maximiliano Sanchez-Lamas, Marcelo J. Yanovsky, Jorge J. Casal and Pablo D Cerdán (2010). Arabidopsis thaliana life without phytochromes. Proc Natl Acad Sci U S A. 107: 4776-4781. PubMed

Roberto J. Staneloni, María José Rodriguez-Batiller, Adamantia Agalou, Pablo D Cerdán, Annemarie H. Meijer, Pieter B. F. Ouwerkerk and Jorge J. Casal (2009). Bell-like homeodomain selectively regulates the high-irradiance response of phytochrome A. Proc Natl Acad Sci U S A. 106: 13624-13629. PubMed

Bárbara Strasser, Mariano J. Alvarez, Andrea Califano and Pablo D. Cerdán (2009). A complementary role for ELF3 and TFL1 in the regulation of flowering time by ambient temperature. The Plant Journal 58: 629-640. PubMed

Amanda C. Wollenberg, Bárbara Strasser, Pablo D. Cerdán and Richard M. Amasino (2008). Acceleration of flowering during shade avoidance in Arabidopsis alters the balance between FLOWERING LOCUS C-mediated repression and photoperiodic induction of flowering. Plant Physiology 148: 1681-1694. PubMed

Sabrina Buchovsky, Bárbara Strasser, Pablo D. Cerdán and Jorge J. Casal (2008). Suppression of pleitropic effects of functional CRYPTOCHROME genes by TERMINAL FLOWER1. Genetics 180: 1467-1474. PubMed

María Agustina Mazzella,Verónica Arana, Roberto J. Staneloni, Susana Perelman, María J. Rodriguez Batiller, Jorge Muschietti, Pablo D. Cerdán, Kunhua Chen, Rodolfo A. Sánchez, Tong Zhu, Joanne Chory and Jorge J. Casal (2005).Phytochrome control of the Arabidopsis transcriptome anticipates seedling exposure to light. Plant Cell 17: 2507-16. PubMed

Cerdán P.D. and Chory, J. Regulation of flowering time by light quality (2003). Nature 423: 881-885. IF2010:36,101. PubMed

Mazzella, A., Cerdán P.D., Staneloni, R.J. and Casal J.J. (2001). Hierarchical coupling of phytochromes and cryptochromes reconciles stability and light modulation of Arabidopsis development. Development 128: 2291-2299. PubMed

Cerdán P.D., Staneloni R.J., Ortega J., Bunge M.M., Rodriguez-Batiller M.J., Sanchez R.A., Casal J.J.(2000). Sustained but not transient phytochrome A signaling targets a region of an Lhcb1*2 promoter not necessary for phytochrome B action. Plant Cell 12:1203-1211. PubMed

Cerdán, P.D., Yanovsky, M.J., Reymundo, F.C., Nagatani, A., Staneloni, R.J., Whitelam, G.C., and Casal, J.J. (1999). Regulation of phytochrome B signaling by phytochrome A and FHY1 in Arabidopsis thaliana. Plant J. 18: 499-507. PubMed

Casal, J.J. , Cerdán, P.D., Staneloni, R.J., Cattaneo,L.L, (1998). Different phototransduction kinetics of phytochrome A and phytochrome B in Arabidopsis thaliana. Plant Physiology. 116: 1533-1538. PubMed

Pablo D. Cerdán
Head of Laboratory -

Sofía Arellano
Estudiante de Licenciatura

Mariana Rosario Barber
Estudiante de Doctorado / Lic. en Biotecnología

Francisco Iglesias
Estudiante de Doctorado

Aimé Jaskolowski
Estudiante de Licenciatura

Christian Damián Lorenzo
Estudiante de Doctorado / Lic. en Cs. Biológicas

Maximiliano Sánchez Lamas
Estudiante de Doctorado / Lic. en Cs. Biológicas